rat igg2aκ isotype control  (Thermo Fisher)

Journal: Stem Cell Reviews and Reports

Article Title: Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine Wound Healing Model

doi: 10.1007/s12015-021-10288-7

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Anti-Mouse Sca-1 , rat IgG2aκ, Clone D7 , APC , Miltenyi Biotec GmbH, Bergisch Gladbach, DE.

Techniques:

Journal: Cells

Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells

doi: 10.3390/cells11091379

Figure Lengend Snippet: The CD38+ cell population expresses hepatic stellate cell specific intracellular markers GFAP and Nestin. CD38 expression of backgated GFAP+ Nestin+ and violet-induced (AF)+ populations after isotype control (upper panel) and antibody (lower panel) directed against CD38. It appeared that the majority of CD38+ cells were positive for Nestin and GFAP and autofluorescent. Proportions (of viable cells) are expressed as a mean ± SD, n = 2.

Article Snippet: Phycoerythrin-cyanine7-conjugated anti-mouse CD38, phycoerythrin-conjugated anti-mouse Nestin, Alexa Fluor 488-conjugated anti-mouse glial fibrillary acidic protein (GFAP), phycoerythrin-cyanine7-conjugated rat IgG2aκ isotype control, phycoerythrin-conjugated mouse IgG2a isotype control, and DRAQ7 were obtained from Thermo Fisher Scientific.

Techniques: Expressing, Control

Journal: Immunology

Article Title: The detection of long‐lasting memory foot‐and‐mouth disease (FMD) virus serotype O‐specific CD4 + T cells from FMD‐vaccinated cattle by bovine major histocompatibility complex class II tetramer

doi: 10.1111/imm.13367

Figure Lengend Snippet: Antibody or staining reagents used in this study

Article Snippet: Rat IgG2aκ isotype control (BD Pharmingen) , R35‐95, IgG2aκ , Alexa 647(BD Pharmingen).

Techniques: Staining

Journal: Immunology

Article Title: The detection of long‐lasting memory foot‐and‐mouth disease (FMD) virus serotype O‐specific CD4 + T cells from FMD‐vaccinated cattle by bovine major histocompatibility complex class II tetramer

doi: 10.1111/imm.13367

Figure Lengend Snippet: Frequency and phenotype of non‐expanded FMDV epitope‐specific CD4 + T‐cell populations ex vivo . Non‐expanded PBMCs were stained with loaded tetramer in combination with a cocktail of mAbs for negative selection (CD8, TCR1‐N24 delta chain, CD14 and CD21), mAbs for the detection of CD4, CD45RO and CCR7, and the fixable viability dye eFluor 780. Data were then acquired by flow cytometry. (a) Dot plots, using data obtained with p220‐loaded tetramer–PE, showing the gating strategy for receptor phenotyping of non‐expanded FMDV‐specific CD4 + T cells ex vivo . (b‐h) The memory phenotype of individual peptide‐specific CD4 + T cells is shown for each animal (FMD7 or FMD9) and peptide‐loaded tetramer. Red dots indicate FMDV peptide‐specific CD4 + T cells in PE‐enriched PBMC aliquots, and grey dots indicate CD4 + ‐gated cells in pre‐enriched PBMC aliquots. The frequency of peptide‐specific CD4 + T cells, per million CD4 + T cells in the respective PBMC population, is shown below each animal‐peptide name. The x‐axis represents CCR7‐Alexa 647 and y‐axis CD45RO‐PerCP/Cy5·5 staining. The ratio of CCR7 to CD45RO double‐positive population in peptide‐specific CD4 + T cells is shown as a red letter in the upper right quantile. Isotype control antibodies (Rat IgG2aκ antibody–Alexa 647 and mouse IgG3 antibody–PerCP/Cy5·5) were used to decide the quantile line. In each quantile compartment, EM denotes effector memory T cells, CM central memory T cells, EF effector T cells and NA naïve T‐cell populations. These dot plots are representative of two independent experiments using two animals

Article Snippet: Rat IgG2aκ isotype control (BD Pharmingen) , R35‐95, IgG2aκ , Alexa 647(BD Pharmingen).

Techniques: Ex Vivo, Staining, Selection, Flow Cytometry